Antioxidant, Cytotoxic Activity and Protein Target Inhibition of Ethyl Acetate Fraction Melinjo Seed (Gnetum gnemon L.) by In Vitro and In Silico Studies on HeLa Cervical Cancer Cells

Cervical cancer is one of the most common suffered in women. Chemotherapy usage often causes physical and psychological side effects patients. This study aims to determine antioxidant cytotoxic ethyl acetate fraction melinjo seeds (Gnetum gnemon L.) on HeLa cervical cells through vitro silico assays. Melinjo seed was extracted by maceration using ethanol 70% fractionated with obtain Ethyl Acetate Fraction Seed (EAFMS). The identification active compounds group done Thin Layer Chromatography (TLC) method. In studies were conducted tests 2,2-diphenyl-1-picrylhydrazyl (DPPH) method activity test 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) Assay. for molecular docking analyzed Autodock Vina TLC analysis EAFMS showed presence stilbenoid group. weak, an IC50 value 175.8 g/ml. Cytotoxic categorized as toxic 21.69 g/ml, while has a synergistic effect combined doxorubicin standard drug combination index (CI) 0.24-0.80. A gnetin C VHR receptor found strong stable bond score -8.3 kcal/mol. Thus, potential be used chemopreventive agent can doxorubicin.